All IL-4 reagents are produced in house and quality controlled, including 10 IL-4 Antibody, 2 IL-4 ELISA, 54 IL-4 Gene, 1 IL-4 Lysate, 6 IL-4 Protein, 3 IL-4 qPCR. All IL-4 reagents are ready to use.
Recombinant IL-4 proteins are expressed by Baculovirus-Insect Cells, E. coli with fusion tags as C-His, Native.
IL-4antibodies are validated with different applications, which are ELISA, WB, ELISA(Cap), ELISA(Det).
IL-4cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each IL-4 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
IL-4ELISA Kit are quality controlled by 8 internation QC standard which guarantee every ELISA Kit with high quality.
Expression host: Baculovirus-Insect Cells
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.