All DDR2 reagents are produced in house and quality controlled, including 5 DDR2 Antibody, 48 DDR2 Gene, 8 DDR2 Lysate, 8 DDR2 Protein, 3 DDR2 qPCR. All DDR2 reagents are ready to use.
Recombinant DDR2 proteins are expressed by HEK293 Cells, Baculovirus-Insect Cells with fusion tags as C-His, N-GST & His, C-cleavage, C-human IgG1-Fc.
DDR2antibodies are validated with different applications, which are ELISA, IHC-P.
DDR2cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each DDR2 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.