All CDK1 reagents are produced in house and quality controlled, including 1 CDK1 Antibody, 28 CDK1 Gene, 1 CDK1 IPKit, 2 CDK1 Lysate, 2 CDK1 Protein, 2 CDK1 qPCR. All CDK1 reagents are ready to use.
Recombinant CDK1 proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-GST & His, N-GST.
CDK1antibodies are validated with different applications, which are WB, ICC/IF, IF, IP.
CDK1cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CDK1 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.