All CD59a reagents are produced in house and quality controlled, including 9 CD59a Antibody, 12 CD59a Gene, 2 CD59a Lysate, 2 CD59a Protein, 1 CD59a qPCR. All CD59a reagents are ready to use.
Recombinant CD59a proteins are expressed by HEK293 Cells with fusion tags as C-human IgG1-Fc, C-His.
CD59aantibodies are validated with different applications, which are ELISA, FCM, IHC-P.
CD59acDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CD59a of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins.It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.