All BTK reagents are produced in house and quality controlled, including 1 BTK Antibody, 28 BTK Gene, 1 BTK IPKit, 1 BTK Lysate, 1 BTK Protein, 2 BTK qPCR. All BTK reagents are ready to use.
Recombinant BTK proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-His, C-His.
BTKantibodies are validated with different applications, which are WB, IHC-P, IP.
BTKcDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each BTK of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.